A colloidal gold immunochromatographic test strip was firstly developed to detect Canine Parainfluenza Virus type 5 based on a sandwich format. The sensitivity of strip was tested for 10^3.8 TCID₅₀ mL-1 to CPIV 5. No cross-reactivity with five common viruses from dogs were observed. Thirty-nine nasopharyngeal swabs samples were used to compare the consistency between RT-PCR and the strip, and the total coincidence rate was 87.18%. The colloidal gold immunochromatographic test strip thus provides a rapid and convenient method for on-site detection of CPIV 5 without any ancillary equipment.

Objective: To establish a stable and reliable model of rabies virus infection by golden hamsters, which can be used to evaluate the protective effects of vaccine prevention, immunoglobulin and antiviral drugs after viral infection. Methods: Golden hamsters were injected intraperitoneally with different concentrations of rabies CVS strain virus to record animal death. Explore the best amount of virus attack and establish a model of the abdominal attack hamster. On this basis, the protective effects of CpG adjuvant rabies vaccine and anti-rabies immunoglobulin on rabies virus challenge were evaluated. Results: When the amount of virus attack remained above 4.1 lgLD₅₀/0.5ml, the mortality of the golden hamster in the control group was 100% within 14 days. Injection of adjuvant vaccine and immunoglobulin after viral infection can significantly reduce the mortality of golden hamsters. Conclusion: The golden hamster abdominal attack rabies virus model was successfully established, and it has simple operation and stable results, which will contribute to the development and screening of antiviral drugs and new adjuvant vaccines.